It was great seing everyone again! We return filled with new ideas and inspiration. Thank you all!

Ann-Sofie, Ewa, Magda, Anders, Oscar and Paul

We are not far from the next ESHRE meeting and we really look forward to seing you all! We will have candy, bags and a small competition every day (with an Ipod for the winner). Visit us at our stand! We will also launch a new product! Are you curious? Just yet another reason to visit our stand no 224-225! Hoping to see you there! Team NidaCon

Last week we returned from a couple of fantastic days in Lyon, France. Our Host; JCD gave us a real treat. We had a wonderful lunch at there beautiful office. We met all of their dedicated and lovely staff and in the evening we where taken out for dinner at a really nice restaurant.

The next day we had the chance to see some of the beautyfull city. Lyon is really worthwile travelling to.

Thank you all at JCD! And a special thanks to Mr. Gilles Morand for your great hospitality.

Best wishes,

Oscar & Anders

Are there any unharmful cryoprotectants or is it all a question of time of exposure!? In that case is perhaps DMSO a good alternative because of the fantastic cryoprotective abilities? Is it maybe that feature we should focus on?

"The provoker"

Ps. Please comment! Ds.

No matter how long the winter, spring is sure to follow.

Spring has sprung on Sweden’s West Coast and the songbirds have returned.  The first flowers are defying gravity and slowly creeping up to meet the sun’s life giving rays and many animals are now giving birth securing a future for their species.

Springtime always brings a feeling of renewed energy and it is important to take a minute and embrace the new life being created around us.  This is exactly how we feel at Nidacon.  With many exciting projects that have been nurtured over the winter we get closer each day to the introduction of new and innovative products.

 

Preparation for this year’s upcoming ESHRE meeting in Amsterdam has begun and we look forward to meeting all who are interested in discussing and learning more about our industry- leading products.  Please feel free to contact us anytime to discuss how our products can be a part of your success.

 

As the winter wind blows, the warm spring sun reminds of summer days not far away!

 

The Nidacon Team

 

 

 

Media for sperm preparations are not in contact with cells for very long and are not really culture media. The products are delivered sterile, making antibiotics unnecessary. In fact, the purpose of using a gradient for sperm is to remove the bacteria (among other things). When preparing sperm with the swim-up technique however, the risk of the sample contaminating the preparation is large, and antibiotics might be needed. This is one of the reasons why Nidacon recommends gradients and not swim-up.

 

Bacterial contamination and sperm recovery after semen preparation by density gradient centrifugation using silane coated silica paticles at different g forces, C.M Nicholson et al, Hum Reprod, (2000),15(4)662-666.

 

The use of two density gradient centrifugation techniques and the swim-up method to separate spermatozoa with chromatin and nuclear DNA anomalies, D Sakkas et al, Hum Reprod, (2000), 15(5)1112-1116.

 

 

 

Media for cell culture may need antibiotics, to prevent bacterial growth in the culture dishes. 

Which should you choose?

 

The most common antibiotics used in cell culture today are:

 

Penicillin, Streptomycin and/or Gentamycin

 

Penicillin

Shelf-life is very short and degradation begins soon after adding to aqueous medium. The degradation products are cell-toxic. Addition of penicillin just before use is best, not during manufacture, not before delivery and not before storage . . .  just before use. 

 

Penicillin degradation products inhibit in-vitro granulopoiesis, Neftel KA et al. Br J Haematol, (1983) 54(2):255-60.

 

Adverse reactions following intravenous pc-g treatment to degradation of the drug in vitro, Neftel et al. Kliniche Wochen Scrhift (1984) 62:25-29

 

Effects of β-Lactam antibiotics on profilerating eukaryotic cells, Neftel et al, Antimicrobial Agents and Chemotherapy (1987) p 1657-1661.

 

 

 

Streptomycin

Streptomycin is an aminoglycoside which can cause hearing and skeletal damage to the foetus. 

 

The antibiotic streptomycin assessed in a battery of in-vitro tests for reproductive toxicology, K. Lemiere et al, Toxicology in Vitro (2007) 21:1348-1353.

 

Aminoglycoside antibiotics mediate context-dependent suppression of termination codons in a mammalian translation system, M Manuvakhova et al, RNA (2000), 6(7):1044-1055, Cambridge University Press.

 

 

Gentamycin

Gentamycin is also an aminoglycoside, classified as a teratogen, not recommended for pregnant women. Gentamycin is nephrotoxic to the foetus, so why use it with gametes or zygotes?

 

An aminoglycoside antibiotic gentamycin induces oxidative stress, reduces antioxidant reserve and impairs spermatogenesis in rats, K Narayana, J. Tox. Sci, (200 , 33(1):85-96.

 

 

Conclusions:

 

If an antibiotic is to be used, the best choice is Penicillin. It is the least toxic if added just prior to use!

 

 

//Anna (Research Coordinator)

Hello ART-field!

What do we say about octoplets? Fertility drugs in combination with IUI? Risk versus success?

"The provoker"

We just returned from a day of seminars in Malaysia, arranged by Sartorius (our distributor in Malaysia) and us NidaCon. We had a great time and it was wonderful meeting with customers on the Malaysian market. Thank you all delegates who honoured us with their attendance!

Malaysia seems to be a beautiful country with beautiful multicultural people and fantastic food! We hope to be able to come back to continue our support of this market.

All of you out there with Chinese origin we would like to take the opportunity of wishing you a happy and prosperous New Year! 

Thank you also, Sartorius, for taking so good care of us and introducing us to the Malaysian and Chinese kitchens fine dining!

Best wishes,

Ann-Sofie & Magda

 

Yes, It's true!

The snow is falling in Utrech! And a few moments later the sun is out and melts everything away.

We have had a wonderful time at the ESDAR-meeting in Utrecht. There have been so many interesting discussions with people from all over the world. We have discussed pigs, cows and horses, as well as sheep and goats. There are so many opportunities and interesting applications for our kind of products. We will have a busy time until next ESDAR.

And yes, we will absolutely take part in next ESDAR in Ghent, Belgium.

 

See you all there!

The Nidacon team