May 12, 2009
2:00 PM
No Phenol red in Nidacon products ...Why?
Phenol red (fenolsulfonftalein) is frequently used in media for cell culture. Its purpose is to ensure the user of the correct pH in the medium. Phenol red has estrogenic effects. Gametes have receptors for estrogen and are thereby affected by its presence.
It is shown that estrogen inhibits sperm motility and acrosome reaction, it also inhibits oocyte maturation.
We have decided to play it safe and therefore do not use phenol red in our media.
Effects of 17 β-estradiol on in vitro maturation of pig oocytes in protein-free medium, Qing Li et al, Journ. of Repr. and Development, (2004), Vol 50, No3.
Impact of estrogenic compounds on DNA integrity in human spermatozoa: Evidence for cross-linking and redox cycling activities, L.E Bennets et al, Mutation Research 641(2008 ) 1-11
Oogenesis in cultures derived from adult human ovaries, A. Bukovsky et al, Repr. Biol. and Endocr. (2005)3:17 doi: 10.1186/1477-7827-3-17,
Estrogenic compounds and oxidative stress (in human sperm and lymphocytes in the Comet assay).Anderson
It is shown that estrogen inhibits sperm motility and acrosome reaction, it also inhibits oocyte maturation.
We have decided to play it safe and therefore do not use phenol red in our media.
Effects of 17 β-estradiol on in vitro maturation of pig oocytes in protein-free medium, Qing Li et al, Journ. of Repr. and Development, (2004), Vol 50, No3.
Impact of estrogenic compounds on DNA integrity in human spermatozoa: Evidence for cross-linking and redox cycling activities, L.E Bennets et al, Mutation Research 641(2008 ) 1-11
Oogenesis in cultures derived from adult human ovaries, A. Bukovsky et al, Repr. Biol. and Endocr. (2005)3:17 doi: 10.1186/1477-7827-3-17,
Estrogenic compounds and oxidative stress (in human sperm and lymphocytes in the Comet assay).
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